There are two major roads from Kampala to Fort Portal, 4WD vehicles are recommended for both:
- Kampala-Fort Portal via Mubende is about 180km, or a 4-5 hour drive, making it the shortest route.
- Kampala-Fort Portal via Masaka, Mbarara and Kasese is longer at 465km (7-8 hrs). This route offers the chance to stop along the way at Lake Mburo National Park, Kyambura Wildlife Reserve, Rwenzori Mountains National Park or Queen Elizabeth National Park.
Semuliki National Park’s Sempaya Gate is 59km from Fort Portal. The park headquarters at Ntandi is 6km further along the road. Historically, the journey was a slow and bumpy 2-3 hour drive on a narrow road that winds over the northern Rwenzori. The route is currently being widened and surfaced to make the journey shorter and more comfortable.